Living in the lawless Ishinari District in a future Japan, sisters Ami (Hina Nagimiya) and Yoshie (Hanakage Kanon) who sold their bodies to help their parents, try to earn a living performing as an idol group in the area. When their performance causes stranger Matsukata (Tak Sakaguchi) a notorious hitman, to notice their condition, the three realize their worth to each other in the fight against Aoyama Dharma (Kimono Negishi), the female boss of a notorious organ harvesting ring in the area. Realizing that even their skills are no match for her army of goons and henchmen, the two are outfitted with special homemade weaponry to their bodies and become the ultimate killers.

Ladies versus Butlers! is a Japanese light novel series written by Tsukasa K?zuki, with accompanying illustrations by Muny?. The series includes 13 novels released between September 2006 and March 2012, published by ASCII Media Works under their Dengeki Bunko imprint. A manga adaptation by the illustrator group Nekoyashiki-Nekomaru was serialized in ASCII Media Works' Dengeki Moeoh magazine between the June 2008 and December 2008 issues. A drama CD based on the novels was released in September 2009. A 12-episode anime adaptation produced by Xebec and directed by Atsushi ?tsuki aired in Japan between January and March 2010. The anime has been licensed by Media Blasters for release in North America.